Transfection:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
Construct:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
Plasmid Preparation:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
Clone Assay:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
Sequencing:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
Software:Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/ .. To investigate the relationship between neuronal activity and mitochondria, we used the genetically encoded calcium indicator GCaMP6s (Addgene plasmid 40753; Douglas Kim) in combination with mitochondrial- DsRed (mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009).
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